Functional analysis of multiple genes related to osteoclastic recognition to bone matrix

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670182
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: National Center for Geriatrics and Gerontology
• Principal Investigator: TAKESHITA SUNAO 国立研究開発法人国立長寿医療研究センター, 国立長寿医療研究センター, 研究室長 (50263009)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 骨代謝 / 骨吸収 / 骨基質

Outline of Final Research Achievements

We found Cthrc1 as a coupling factor which is transcriptionally up-regulated by bone resorption. Then, to define a mechanism in which genes are regulated by bone resorption in osteoclasts, we screened genes regulating by bone matrix and calcium by a microarray. We successfully identified Itgb3, Calcr and Atf3/7, which are highly increased in osteoclasts cultured in the presence of high calcium. Enforced expression of Atf3/7 in osteoclasts increased the expression of Cthrc1, Itgb3 and Calcr mRNA, suggesting that the expression of Atf3/7 induced by bone resorption up-regulates both bone resorbing function and bone formation by a coupling mechanism.

Free Research Field

骨細胞生物学、骨代謝学