2015 Fiscal Year Final Research Report
Distribution of DNA adducts in human tissues and its pathological significance
Project/Area Number |
26670187
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Keywords | DNA 付加体 / アダクトミクス / ゲノミクス / 発がん / 環境発がん / リスク / 暴露評価 / 分子病理疫学 |
Outline of Final Research Achievements |
In this study, mass/charge value and liquid chromatography retention time of known 174 DNA addcuts were incoporated into LC-MS/MS machine (4000-QTRAP; Thermo). Using this adductome approach, three to 10 sites/case of gastric mucosa (31 cases) were investigated for DNA adducts measurements. Considerable numbers of known DNA adducts were present in human gastric mucosa in different quantities. Among them, tabacco-related adducts, lipid-peroxidation-induced DNA adducts, oxidative stress and alcohol related adducts, PhIP adduct was also noted. Tabacco-related adducts of current-smokers obviously increased as compared with that of non-smoker, but that of ex-smokers do not increase. It is assumed that tabacco-related adducts of ex-smoker diminish by epithelial cell turnover of the stomach. In the lung, adductome profile was different between smokers' and never smokers' lung; between lungs having adenocarcinoma and squamous cell carcinoma of smokers.
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Free Research Field |
腫瘍病理学
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