Search for RNA Biomarker in Aqueous Humor from patients by Conprehensive Transcriptome Analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670263
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied pharmacology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Tanaka Yuji 国立研究開発法人理化学研究所, 情報基盤センター, センター研究員 (40625513)
• Co-Investigator(Kenkyū-buntansha): 河合 純 国立研究開発法人理化学研究所, 予防医療・診断技術開発プログラム, 副プログラムディレクター (30391923) ; 國方 彦志 東北大学, 医学系研究科, 准教授 (40361092) ; 藤田 幸輔 東北大学, 医学系研究科, 助手 (80708115)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: circulating miRNA / 前房水 / 網羅的解析 / 次世代シーケンサー

Outline of Final Research Achievements

We extracted a trace amount of RNA from anterior aqueous humor from patients with ophthalmopathy of just a few 100 μL and tried comprehensive RNA analysis using next generation sequencer etc. About 360 kinds of microRNAs were successfully detected from one clinical specimen, and about one third of them were commonly detected in eight cataract specimens. Of these, 75% of the top 40 in expression levels were consistent with other studies reported during the study period. As a study that tried comprehensive analysis individually without mixing samples, our microarray analysis reported at the beginning of the research period is the first time, and the analysis scale by the next generation sequencer is the largest. In addition, as a surrounding study, we have examined microRNA analysis method and pathology model evaluation method and obtained basic knowledge.

Free Research Field

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