Efficient induction of in vivo RNA interference by designing exosome-tropic siRNA derivatives

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670266
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied pharmacology
• Research Institution: Kyoto University
• Principal Investigator: Takahashi Yuki 京都大学, 薬学研究科(研究院), 助教 (00547870)
• Co-Investigator(Kenkyū-buntansha): TAKAKURA Yoshinobu 京都大学, 大学院薬学研究科, 教授 (30171432) ; NISHIKAWA Makiya 京都大学, 大学院薬学研究科, 准教授 (40273437)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: エキソソーム / ラジオアイソトープ / DDS

Outline of Final Research Achievements

In the current study, for the goal of the development of drug delivery system by using exosomes, a method to quantitatively evaluate in vivo tissue distribution of exosomes was developed, and three ultracentrifugation-based exosome collection methods were compared to one another. We selected a biotin binding protein (streptavidin: SAV) and an exosome-tropic protein (lactadherin: LA) to obtain a fusion protein SAV-LA. Radio-labeled exosomes were prepared by using SAV-LA and radioactive biotin derivatives, and tissue distribution of the exosomes was quantitatively evaluated by using the labeled exosomes. By comparing the three ultracentrifugation-based exosome collection methods, we concluded that a density gradient ultracentrifugation method was the best method to collect exosomes with minimal aggregates and in a good quality.

Free Research Field

生物薬剤学