2015 Fiscal Year Final Research Report
Establishment of ELISA that detect polymeric OPN and its usefulness
| Project/Area Number |
26670280
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Nishimichi Norihisa 広島大学, 保健管理センター, 研究員 (00583486)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | オステオポンチン / 重合 / ELISA / 好中球 |
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| Outline of Final Research Achievements |
To establish a new ELISA that detects polymeric osteopontin, which we previously found as a chemoattractant for neutrophils, we attempted to obtain polymeric OPN specific monoclonal antibodies. First we generated mice that express only polymerization-incompetent OPN by introducing some mutations into the OPN gene. The mice produced many antibody clones responding to injected polymeric OPN. However, many of clones recognized wild type OPN, which prevent us from establish the specific clones. Probably, immunological tolerance was not sufficient for the mice not to respond wild type OPN. We restarted to generate the tolerant mice by CRISPR-Cas9 method.
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| Free Research Field |
呼吸器内科学、分子病態学
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