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2015 Fiscal Year Final Research Report

Establishment of evaluation method of drugs using a megakaryocytic cultured cells.

Research Project

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Project/Area Number 26670282
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionThe University of Tokushima

Principal Investigator

HOSOI Eiji  徳島大学, 大学院医歯薬学研究部, 教授 (70229186)

Co-Investigator(Kenkyū-buntansha) AKI Kensaku  徳島大学, 大学院医歯薬学部研究部, 助教 (70646398)
Project Period (FY) 2014-04-01 – 2016-03-31
Keywords小板 / 巨核球系培養細胞 / Ca2+ / PMA / 分化誘導 / HEL / CMK / 薬剤評価法
Outline of Final Research Achievements

In the present study, we attempted to improve the evaluation of various drugs on the platelet functions using PMA-induced megakaryocytic HEL cells and CMK cells as an alternative cell of platelets. We selected aspirin and cilostazol as antiplatelet drugs and ibuprofen and sodium valproate as other drugs. In conclusion, PMA-induced megakaryocytic HEL cells and CMK cells are a useful model for this study of platelet functions, and the quantification of inhibition of thrombin-induced [Ca2+]i and thrombin-induced CD62P antigen were applicable to the evaluation of various drugs on platelets. Furthermore, this evaluation system using these platelet model cells may contribute to appropriate drug selection and doses in order to control of the platelet functions without using the platelet, and is expected future use in clinical field.

Free Research Field

輸血検査学,免疫検査学

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Published: 2017-05-10  

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