Development of Novel Rapid Identification Test Method of Nosocomial Pathogen clones using MALDI-TOF Measurement

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670350
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical and hospital managemen
• Research Institution: International University of Health and Welfare
• Principal Investigator: NAGASAWA ZENZO 国際医療福祉大学, 保健医療学部, 教授 (80706820)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 疫学解析 / MRSA / 緑膿菌 / MALDI-TOF MS / PFGE法 / POT法 / REP-PCR法 / リボゾーム蛋白

Outline of Final Research Achievements

MALDI Biotyper 3.1 and ClinPro Tools ver.2.2 were used and measurement data for MRSA, Pseudomonas aeruginosa and Clostridium difficile was analyzed in this study. We could originally develop novel test method(IUHW method) using MALDI-TOF MS and clone typing data analysis with epidemiological auto-classification Good relationship with IUHW method was obtained between PFGE, POT, REP-PCR for MRSA. But relationship discrepancy with IUHW method was observed in some clones for P. aeruginosa. Cluster classification was not achieved for C. difficile between PCR-ribotyping and ClinPro Tools. We think it is possible to achieve rapid classification of clones using MALDI-TOF MS by development of optimal measurement conditions and continuous study has been conducted.

Free Research Field

臨床微生物学