2016 Fiscal Year Final Research Report
Study on molecular pathology in sudden death due to Excited Delirium
| Project/Area Number |
26670354
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OUCHI Tsukasa 東北大学, 医学系研究科, 技術員 (90712266)
HASHIYADA Masaki 関西医科大学, 医学部, 准教授 (40374938)
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| Research Collaborator |
HOSOYA Tadashi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 興奮性譫妄 / microRNA / 拘束 / 実験モデル / マイクロアレイ / 次世代シークエンス |
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| Outline of Final Research Achievements |
MicroRNAs (miRs) are increasingly associated with various diseases and biomechanisms, we studied serum miRs to find physical stress markers.To demonstrate changes in serum and cardiac miR levels when physical stress is applied, we constructed three stress modalities using rats: alcohol intake, treadmill running and restraint. As methods of miR measurement, qRT-PCR was performed after a microarray with serum. In addition, a comprehensive analysis using next-generation sequencing was performed in myocardium. This study showed that there are miRs whose expression level changes due to constraint stress after exercise.It was also suggested that different miRs were expressed depending on the different stress.
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| Free Research Field |
法医学
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