Detection of autophagy in human skin wounds and its application for wound age detemination

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670357
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: Ishida Yuko 和歌山県立医科大学, 医学部, 講師 (10364077)
• Co-Investigator(Kenkyū-buntansha): KIMURA Akihiko 和歌山県立医科大学, 医学部, 准教授 (60136611)
• Research Collaborator: KUNINAKA Yumi ; YAMAMOTO Hiroki
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 創傷治癒

Outline of Final Research Achievements

The dorsal skin of Balb/c wild-type mice were wounded with a diameter of 4 mm biopsy punch. Gene and protein expressions of LC3 were significantly reduced at 1 day after the injury compared with unwounded skin. On the other hand, gene and protein expressions of p62 were significantly increased at 1 day after the injury compared with unwounded skin. Moreover, protein expression of LC3 and p62 were found in F4/80-positive macrophages on the tissue sections. Collectively, macrophages might be activated after injury along with autophagy inhibition, therefore, autophagy detection can be a useful marker for wound age determination.

Free Research Field

実験病理