An experimental trial to determine the presence and absence of antemortem seizure using nuclear magnetic resonance analysis of rat postmortem cerebrospinal fluid

2014 Fiscal Year Final Research Report

• Project/Area Number: 26670359
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Nippon Medical School
• Principal Investigator: HIRAKAWA KEIKO 日本医科大学, 医学部, 助教 (30165162)
• Co-Investigator(Kenkyū-buntansha): KOIKE Kaoru 京都大学, 医学（系）研究科(研究院), 教授 (10267164)
• Co-Investigator(Renkei-kenkyūsha): KANAWAKU Yoshimasa 日本医科大学, 医学（系）研究科(研究院), 講師 (80465343) ; MORIYAMA Tsuyoshi 東京工芸大学, 工学部, 准教授 (80449032) ; OHNO Youkichi 日本医科大学, 医学（系）研究科(研究院), 教授 (70152220) ; KANETAKE Jun 防衛医科大学校（医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部医学科専門課程, 教授 (90326661)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: けいれん / 死後髄液 / 核磁気共鳴法 / パターン認識 / 死体検案

Outline of Final Research Achievements

We performed nuclear magnetic resonance (NMR) analysis for rat postmortem cerebrospinal fluid (CSF) and ascertained if presence and absence of antemortem seizure could be detected. Male Sprague-Dawley rats (9 weeks, 220 g) were randomized into four groups. 1) seizure group (n=13); saline (12mL/kg) ip + pentylentetrazole (PTZ) (70 mg/kg) ip 6 hrs later, 2) seizure suppression group (n=10); pentobarbital (PB) (90mg/kg) ip + PTZ ip 6 hrs later, 3) anticonvulsant alone group (n=13); PB ip + saline (1.75mL/kg) ip 6 hrs later, 4) control group (n=9); saline + saline ip 6 hrs later. After animals were euthanized by CO2 gas, CSF samples collected in the cisternal compartment were analyzed by proton NMR spectroscopy. The NMR spectral profile of 4 groups showed separation and clustering on a PCA score plot.

Free Research Field

法医学