Effects of psychotropic agents and low temperature on adipocyte and adipose tissue

2017 Fiscal Year Final Research Report

• Project/Area Number: 26670360
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: Fujita Health University
• Principal Investigator: ISOBE Ichiro 藤田保健衛生大学, 医学部, 教授 (30315907)
• Co-Investigator(Renkei-kenkyūsha): OCHI Hiroshi 藤田保健衛生大学, 医学部, 助教 (70527704) ; HIRATA Yukari 藤田保健衛生大学, 医学部, 助教 (50156676) ; HAMAJIMA Makoto 藤田保健衛生大学, 医学部, 助教 (20189608)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 脂肪細胞 / 心筋細胞 / 内因性カンナビノイドシステム

Outline of Final Research Achievements

Our purpose was to investigate the roles of adipocyte on the pathophysiology of the cardiovascular system and to clarify the mechanisms underlying serious pathological outcome including sudden death and to find out biomarkers that indicate abnormal conditions of adipocyte. To achieve our purpose, we tried to prepare a culture system model of adipocyte using 3T3-L1 cell line. This cell line has been utilized widely as an adipocyte model, but the 3T3-L1 cell have originally fibroblast-like phenotype and have to be differentiated into adipocyte-like phenotype by a couple of reagents. Moreover, it is difficult to maintain the adipocyte-like phenotype for a long time in a culture system. We, therefore, tested various conditions of cell culture, and we recognized that the number of cell passage, cell confluency, or timing of addition or removal of differentiation reagents were important factors for establishing a stable culture system.

Free Research Field

医歯薬学　法医学　法医病理学　神経生化学　細胞生物学