Establishment new HCC derived cell lines that support efficient hepatitis virus replication.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670378
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kanazawa University
• Principal Investigator: HONDA Masao 金沢大学, 保健学系, 教授 (00272980)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA Taro 金沢大学, 附属病院, 助教 (90377432) ; SHIMAKAMI Tetsuro 金沢大学, 附属病院, 助教 (50436820) ; SHIRASAKI Takayoshi 金沢大学, 保健学系, 助教 (50547180)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ウイルス培養細胞 / 幹細胞マーカー / 自然免疫 / 癌 / 感染症

Outline of Final Research Achievements

We established a new cell line that is positive for cancer stem cell marker, EpCAM and supports efficient HCV replication and infection (JFH-1 and HJ3-5), from HCC tissue infected with genotype 1b HCV. KH cells have intact RIG-I sequence and produce IFN signaling, therefore, HCV replication once decline and increased again after infection. TP53 status of KH is wild and established tumor formation in NOD-SCID mice. Thus, we established a new HCC cell line that support efficient HCV replication and infection. Further studies should be needed to establish KH derived cells that support an efficient genotype 1b HCV replication.

Free Research Field

肝臓病学