2015 Fiscal Year Final Research Report
The purification and expansion of human iPS cell-derived intestinal precursor cells for future application in regenerative medicine
Project/Area Number |
26670384
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
|
Research Institution | Tokyo Institute of Technology |
Principal Investigator |
Kume Shoen 東京工業大学, 大学院生命理工学研究科, 教授 (70347011)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | 腸上皮細胞 / 分化誘導 / 多能性幹細胞 |
Outline of Final Research Achievements |
We established a cost effective protocol to induce endoderm differentiation with low concentration of Activin by adding DMSO. We found that at the presence of DMSO, the threshold of Activin to induce intestinal differentiation is much reduced. We also established a protocol to selectively culture the human iPS cells-derived intestinal epithelial cells from a mixed cell population, so that the purified cells could be expanded and used for further studies. We also found that using defined growth factors and chemicals, the purified cells could be expanded and further matured. The present studies therefore enabled us to prepare a certain amount of cells that could be used for further studies for establishing regenerative medicine of the intestine.
|
Free Research Field |
幹細胞生物学
|