Development of a novel therapeutic strategy targeting genes contributing to forming cluster in EGFR mutated lung cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670416
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Nagoya University
• Principal Investigator: Sato Mitsuo 名古屋大学, 医学部附属病院, 講師 (70467281)
• Co-Investigator(Kenkyū-buntansha): KONDO Masashi 名古屋大学, 大学院医学系研究科, 准教授 (00378077) ; HASE Tetsunari 名古屋大学, 医学部附属病院, 病院助助 (30621635)
• Co-Investigator(Renkei-kenkyūsha): YUKAWA Hiroshi 名屋大学, 大学院工学系研究科, 特任講師 (30634646)
• Research Collaborator: YOGO Naoyuki 名古屋大学, 大学院医学系研究科, 博士過程3年生
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: EGFR遺伝子変異 / RNA干渉 / プールshRNA

Outline of Final Research Achievements

This study is aimed to test the hypothesis that EGFR mutated lung cancer cells complete a metastatic process by maintaining epithelial characteristics through expressing adhesion molecules. To identify genes contributing to forming clustering, we performed a screen with pooled shRNA library. The library was lentivirally transduced in immortalized normal bronchial epithelial cell lines (HBEC) and then the HBEC were grown in 2D or 3D culture condition. Comparing amount of shRNA between the two conditions by next generation sequencing, we identified potential genes contributing to forming cluster. To confirm this result, we also did individual knockdown of the identified genes. Through these experiments, we were able to identify genes that could serve as therapeutic target in EGFR mutated lung cancer.

Free Research Field

呼吸器内科