2015 Fiscal Year Final Research Report
Development of the methodology to evaluate oxygenation of organs in vivo utilizing organic luminescence
| Project/Area Number |
26670425
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 低酸素 / 腎臓 / りん光プローブ |
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| Outline of Final Research Achievements |
Previous methods to evaluate oxygenation of organs measured oxygen tensions in the blood stream of organs, and no method could evaluate intracellular oxygenation. We employed the lipophilic phosphofluorescence probe, which is quickly taken up by tubular cells in the kidney. Utilizing this probe, we established the method to measure intracellular oxygen tensions accurately by calculating half-life of the probe. This method allowed us to prove changes of intracellular oxygen tensions of tubular cells in a variety of disease models. Hypoxia is the final common pathway of chronic kidney disease, and this method will serve as a powerful tool to develop novel drugs against kidney disease.
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| Free Research Field |
酸素生物学
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