Analysis of RNA binding proteins in polyglutamine disease

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670445
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Yokohama City University
• Principal Investigator: TANAKA Fumiaki 横浜市立大学, 医学(系)研究科(研究院), 教授 (30378012)
• Co-Investigator(Kenkyū-buntansha): DOI Hiroshi 横浜市立大学, 医学部, 講師 (10326035) ; KOYANO Shigeru 横浜市立大学, 医学部, 准教授 (50315818) ; TADA Mikiko 横浜市立大学, 附属病院, 助教 (30722467)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ポリグルタミン病 / PCBP

Outline of Final Research Achievements

Using aggregate proteome analysis we identified many component proteins of polyglutamine nuclear aggregates including RNA binding proteins. In this study we analyzed the staining patterns of PCBP1, PCBP2, PCBP3, hnRNPU, hnRNP H1, hnRNP H2, hnRNP F, DDX5, DDX17, Matrin 3, and SGTA in human autopsy brains of various polyglutamine diseases. As a result, Matrin 3 and SGTA were observed in nuclear aggregates. Preliminary result shows that SGTA suppresses aggregate formation in Huntington's disease cell model. Further, we identified SGTA-binding proteins using mass spectrometry.

Free Research Field

神経内科学