Functional analysis of symmetric division-related molecules in the regulation of self-renewal of hematopoietic stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670471
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Kyushu University
• Principal Investigator: ARAI FUMIO 九州大学, 医学(系)研究科(研究院), 教授 (90365403)
• Research Collaborator: MacArthur Ben D. 英国、サウサンプトン大学
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 造血幹細胞 / 自己複製 / 細胞分裂

Outline of Final Research Achievements

The number of hematopoietic stem cells (HSCs) is controlled by the balance of the asymmetric/symmetric divisions. We have identified that niche factor Angpt1 increases the symmetric gene expression in paired daughter cells (PDCs) and induces self-renewal division of HSCs. To clarify the function of Angpt1 in the regulation of self-renewal division, we are investigating the function of Bmi1 that is induced symmetric expression between daughter cell pairs by Angpt1 in the regulation of self-renewal of HSCs.
In this study, we also analyzed whether Angpt1 could induce the deterministic HSC self-renewal. We identified that Angpt1-induced self-renewal fitted to the stochastic model in which individual divisions were not closely regulated, regulation was exerted at the level of probabilities. We also analyzed the effect of Angpt1 on cell division induced HSC aging. We found that the culture induced age-related phenotype in PDCs and Angpt1 prevented the cell division induced aging of HSCs.

Free Research Field

幹細胞生物学