Studies of autoimmune myositis using iPS cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670474
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Kohsaka Hitoshi 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (00251554)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 多発性筋炎 / 皮膚筋炎 / iPS / 自然免疫

Outline of Final Research Achievements

To investigate pathogenesis of polymyositis and dermatomyositis, intrinsic abnormality of the patient muscles has to be studied. From peripheral blood from the patients, we have established human iPS cells that express the MyoD protein inducible with doxycycline. When doxycycline was added in the culture medium, the cells became spindle-like cells and gained characteristics of muscle cells. They produced a wide variety of inflammatory cytokines that are produced reportedly from regenerating muscle cells.

Free Research Field

膠原病・リウマチ内科