Gene-based analysis of NETs-associated DNA in autoantibody-mediated diseases

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670519
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Chiba University
• Principal Investigator: MATSUOKA YUMI 千葉大学, 医学(系)研究科(研究院), 助教 (10402067)
• Co-Investigator(Kenkyū-buntansha): KAMBE Naotomo 千葉大学, 大学院医学研究院, 准教授 (50335254)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 皮膚科学 / 血管炎 / エピジェネティクス

Outline of Final Research Achievements

First, we tried to collect neutrophils from normal human peripheral blood. However, there were some technical difficulties about collecting naive neutrophils from human blood. Therefore, we tried to obtain mouse naive neutrophils from bone marrow and analyzed ROS production, NETs release and antimicrobial peptide gene expressions under some stimulative conditions. We successfully isolated naive neutrophils from mouse bone marrow and visualized NETs release under C.albicans stimulation. We will establish the new method for visualizing NETs release and perform gene-based analysis of NETs-associated DNA in autoantibody-mediated diseases in our future project.

Free Research Field

皮膚科学