Study of a highly sensitive C-13 MR glucose metobolite mapping with the brain of an Altzheimer model mouse

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670554
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Morikawa Shigehiro 滋賀医科大学, 神経難病研究センター, 客員教授 (60220042)
• Co-Investigator(Kenkyū-buntansha): YANAGISAWA Daijiro 滋賀医科大学, 神経難病研究センター, 准教授 (50581112)
• Co-Investigator(Renkei-kenkyūsha): TOOYAMA Ikuo 滋賀医科大学, 神経難病研究センター, 教授 (20207533)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アルツハイマー病 / APPマウス / 脳代謝画像 / グルコース代謝 / 13C-NMR

Outline of Final Research Achievements

To investigate Alzheimer's disease from the aspect of glucose metabolism in the brain, 13C NMR metabolite mapping in APP/PS1 mouse brain was observed after the infusion of 13C glucose. For highly sensitive data acquisition, 1H-detected 13C NMR spectroscopic imaging was utilized with a 7T MR system, 100G/cm gradient system and Tx/Rx separated RF coil system. 13C Glu/Gln signals of the brain increased to the maximal level at 2 hours after 13C glucose infusion, and then the signals gradually decreased in either APP/PS1 mice or wild type mice at the age of 18 months. Apparent differences in the signals between the two groups wwere not recognized, since natural abundant big 13C signals from fat tissue around the skull disturbed the observation of signals from the brain and variations among the experiments were big. Specific 13C signals of the brain in APP/PS1 mice were not detected.

Free Research Field

磁気共鳴医学