Transplantation of islets in immune tolerance site under skin induced by bFGF or SEK-1005

2014 Fiscal Year Final Research Report

• Project/Area Number: 26670577
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: IWATA Hiroo 京都大学, 再生医科学研究所, 教授 (30160120)
• Co-Investigator(Kenkyū-buntansha): HAMAGUCHI Masahide 京都府立医科大学, 医学部附属病院, 専攻医 (80350883)
• Co-Investigator(Renkei-kenkyūsha): SAKAGUCHI Shimon 大阪大学, 免疫学フロンティア研究センター, 教授 (30280770)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: ランゲルハンス氏島 / 膵島 / 制御性T細胞 / 糖尿病 / 血糖値 / 移植 / 皮下移植

Outline of Final Research Achievements

We demonstrated that blood glucose levels of diabetic rats were kept stable normal by transplanting islet at subcutaneous sites without immunosuppressive drug. Transplantation sites were prepared by implanting agarose rods with SEK-1005 or bFGF. The population of regulatory T (Treg) cells infiltrated into granulomatous tissue formed around the agarose rods were significantly increased in comparison with normal subcutaneous tissue. The population of Treg cells were kept constant higher levels after islet transplantation. Gene expression of the granulomatous tissue around agarose rods 7 days after implantation were analyzed. The expression levels of Treg cells associated chemokines and cytokines were increased in granulomatous tissue. Concentration of activated TGF-β1 in exudate reached maximum 10 days after implantation of agarose-SEK. The immune tolerance site was formed around the agarose rods.

Free Research Field

医歯薬学