Development of Isolated Mitochondria Transplantation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670586
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Watanabe Taiji 京都府立医科大学, 医学(系)研究科(研究院), 助教 (20448723)
• Research Collaborator: GOJO Satoshi 京都府立医科大学, 医学研究科, 教授 (90316745) ; TABATA Yasuhiko 京都大学, 生体組織工学研究部門生体材料学分野, 教授 (50211371) ; KAMI Daisuke 京都府立医科大学, 医学研究科, 講師 (80415588)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ミトコンドリア / 移植 / 心筋細胞

Outline of Final Research Achievements

It is known that the efficiency of mitochondria transfer into cells in primary cultured cells is low efficiency. Therefore, we focusing on TAT peptide known as the cell membrane penetrating peptide, we have constructed a system that can high-efficiently transfer mitochondria into primary cultured cells. We introduced mitochondria using this TAT dextran and showed that the introduction efficiency of mitochondria improves on rat neonatal cardiomyocytes (RNCM), and it reduces the apoptosis induction rate of hydrogen peroxide treated RNCM. In the future, we will evaluate the mitochondrial function using the oxygen consumption rate as an indicator for mitochondrial activity of RNCM introduced mitochondria.

Free Research Field

心血管移植