2015 Fiscal Year Final Research Report
Development of imaging for solid tumors using indocyanine green incorporated into nanoparticles.
| Project/Area Number |
26670601
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | ナノ粒子 / 固形腫瘍 / インドシアニングリーン (ICG) / イメージング |
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| Outline of Final Research Achievements |
We successfully formed up ICG-incorporated nanoparticles of carbonate apatite (ICG-CA) with the size of 20nm in diameter. ICG-CA gave 1.5-2 folds higher uptake of ICG in colon cancer cells in vitro as compared to ICG alone. In vivo imaging study also revealed that intra-venous administration of ICG-CA particles enhanced uptake of ICG fluorescence for the subcutaneous tumors in nude mice, when compared to systemic injection of ICG alone. CA particles also enhanced ICG uptake into xenograft tumors when ICG alone was injected into intra-abdominal space and CA was injected via tail vein almost at the same time. We found that CA reduced integrin β1 expression and it lowered tumor interstitial fluid pressure, which may in part explain the underlying mechanism.
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| Free Research Field |
消化器外科
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