2015 Fiscal Year Final Research Report
Role of epigenetic modification by cancer reprograming in colon tumorigenesis model
| Project/Area Number |
26670605
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
Yamamoto Hirofumi 大阪大学, 医学(系)研究科(研究院), 教授 (30322184)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | microRNA / miR-200c / miR-369 / miR-302 / CPC/Apc mouse / MAF / P53 |
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| Outline of Final Research Achievements |
MicroRNA (miR) is an attractive modulator in the field of cancer research, and it is known that its aberrant expression causes dysregulation of cancer-related genes. We previously reported that introduction of miRs (miR-200c, 302, 369) in colon cancer cells displayed an ability to change epigenetic profiles to the pluripotent state, and inhibited malignant features, including cell proliferation, chemoresistance, and tumorigenicity. In this study, we found that administration of the miRs suppressed the incidence of tumors in vivo in the course of tumorigenesis of CPC/Apc mouse, a mouse model of adenoma-carcinoma progression. In addition, these miRs increased expression of MAF gene, an inducer of apoptosis-related gene p53 in the normal mucosa. In vitro experiments supported the hypothesis that MAF behaved as a tumor suppressor in the p53-dependent mechanism.
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| Free Research Field |
消化器癌
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