Basic researches for the improvement of the fragility and the organ portection about de-and re-cellularization based regenerated lung

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670629
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: Nagasaki University
• Principal Investigator: TSUCHIYA Tomoshi 長崎大学, 医歯薬学総合研究科(医学系), 准教授 (30437884)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 肺再生 / 肺 / 再生 / 組織工学 / 脱細胞化 / 再細胞化 / 肺移植 / マトリックス

Outline of Final Research Achievements

Decellularization and recellularization technique is the most useful for lung regeneration. The aim of the present study was to regenerate lung using the technique and the main subjects were improvement of the decellularization method including decellularization route and decellularization solution. As results, we developed the NaOH based decellularization solution; the matrix damage was similar to original method and the total cost of the method was reduced. We could also developed ventilation-based decellularization system of the lung. The live imaging of the recellularization represented that adipose tissue derived stromal cells migrated stroma and differentiated into pericytes.

Free Research Field

再生医学　呼吸器外科学