Strategy for epithelial thymic malignancy treatment focusing on L-type amino acid transporter 1

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670631
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: Dokkyo Medical University
• Principal Investigator: CHIDA MASAYUKI 獨協医科大学, 医学部, 教授 (70333812)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Keitaro 獨協医科大学, 医学部, 准教授 (10323106)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 胸腺癌 / 胸腺腫 / アミノ酸トランスポーター / LAT1

Outline of Final Research Achievements

To evaluate the effect of L-type amino acid transporter 1 (LAT1) and cMyc-LAT1 axis in thymic carcinoma, we analyzed the function of LAT1 in Ty82 human thymic carcinoma cells. LAT1 was detected in Ty82 cells by Western blot. A LAT1-specific inhibitor, JPH203, reduced the cell growth of Ty82. JPH203 promoted the dephosphorylation of S6 kinase and phosphorylation of eukaryotic initiation factor 4E-binding protein 1, indicating that a LAT1-specific inhibitor inactivates mTOR, and leading to the suppressionof Ty82 cell proliferation. LAT1 was detected in human thymic carcinoma specimens, but not in those of thymoma.

Free Research Field

医学　胸部外科学　呼吸器外科学