2015 Fiscal Year Final Research Report
Strategy for epithelial thymic malignancy treatment focusing on L-type amino acid transporter 1
Project/Area Number |
26670631
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Respiratory surgery
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Research Institution | Dokkyo Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
HAYASHI Keitaro 獨協医科大学, 医学部, 准教授 (10323106)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Keywords | 胸腺癌 / 胸腺腫 / アミノ酸トランスポーター / LAT1 |
Outline of Final Research Achievements |
To evaluate the effect of L-type amino acid transporter 1 (LAT1) and cMyc-LAT1 axis in thymic carcinoma, we analyzed the function of LAT1 in Ty82 human thymic carcinoma cells. LAT1 was detected in Ty82 cells by Western blot. A LAT1-specific inhibitor, JPH203, reduced the cell growth of Ty82. JPH203 promoted the dephosphorylation of S6 kinase and phosphorylation of eukaryotic initiation factor 4E-binding protein 1, indicating that a LAT1-specific inhibitor inactivates mTOR, and leading to the suppressionof Ty82 cell proliferation. LAT1 was detected in human thymic carcinoma specimens, but not in those of thymoma.
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Free Research Field |
医学 胸部外科学 呼吸器外科学
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