Maintenance of homeostasis for intervertebral disc by cell cycle-related genes

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670651
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Hokkaido University
• Principal Investigator: Sudo Hideki 北海道大学, 医学(系)研究科(研究院), 准教授 (30374367)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 椎間板変性 / 細胞周期

Outline of Final Research Achievements

Although human intervertebral disc (IVD) degeneration can cause several spinal diseases, its pathogenesis remains unclear. This study aimed to create a highly reproducible in vivo mouse IVD degeneration model. One hundred six mice were operated and L4/5 IVD was stabbed with a 35 or 33-gauge needle. Micro-computed tomography scanning was performed, and the stabbed region was confirmed. Evaluation was by magnetic resonance imaging (MRI) and histology using our classification scoring system. In IVDs stabbed with either needle, significant degeneration occurred relative to the control. Our histological classification scores correlated well with MRI findings and could detect degenerative progression, irrespective of the stabbed region.In this study, a highly reproducible in vivo mouse IVD degeneration model was created with precise information about surgical techniques.

Free Research Field

整形外科