2016 Fiscal Year Final Research Report
Establishment of a novel method for heat-stimulus induced osteogenesis using magnetite nanoparticles
| Project/Area Number |
26670659
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 温熱療法 / 骨形成 / 動物モデル / 人工骨 / 骨欠損 |
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| Outline of Final Research Achievements |
New bone formation was significantly increased by hyperthermia using magnetite cationic liposome embedded in alginate beads with an alternating magnetic field (AMF) in a rat tibial defect model. Heating materials were changed to Resovist containing ferucarbotoran and hydroxyapatite scaffold, which have been already approved in clinical use in Japan. Combination materials were grafted in tibial defect of rat and rabbit and subjected to the multiple hyperthermia in AMF. Results demonstrated that newly formed bone was observed in the grafted materials from the periphery toward the center, and more osteoclasts were found in the once a week hyperthermia group compared with non-hyperthermia group.
Experiments of hyperthermia were performed in vitro using ATDC-5 and MC3T3 cells. Heat stress also induced enhanced alkaline phosphatase expression in cultured osteoblastic cells, MC3T3. On the other hand, heat stress had no obvious effects on chondrogenic differentiation using ATDC5 cells.
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| Free Research Field |
整形外科学
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