Screening of small compounds and factors to re-differentiate dedifferentiated chondrocytes in 2D culture

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670662
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: FUJITA KAORI 京都大学, iPS細胞研究所, 特定研究員 (10633092)
• Co-Investigator(Renkei-kenkyūsha): TSUMAKI Noriyuki 京都大学, iPS細胞研究所, 教授 (50303938) ; OHTA Akira 京都大学, iPS細胞研究所, 教授 (00168931)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 低分子化合物 / 軟骨細胞 / 脱分化 / 再分化

Outline of Final Research Achievements

We performed a small molecular weight compounds screen to identify compounds which re-differentiate dedifferentiated chondrocytes in monolayer-culture. A compound library consists of over 18,200 compounds introduced into the chondrogenic cell line (290-2-14)), which were directly converted from dermal fibroblasts of Col11a2-Egfp-Ires-Puro transgenic mice by inducible expression of c-Myc, Klf4 and Sox9 with doxycycline (Dox). We identified one candidate compound and analyzed its ability to re-differentiate dedifferentiated chondrocytes. Treatment of dedifferentiated chondrocytes with the candidate compound dose-dependently up-regulates chondrogenic marker genes (Col2a1 and Col11a2). Now, we have analyzed the detail molecular mechanisms for re-differentiation ability of the candidate compound.

Free Research Field

分子細胞生物学