2015 Fiscal Year Final Research Report
In vitro reconstruction and spatiotemporal analysis of the cellular network in bone metabolism using fluorescence technology
| Project/Area Number |
26670667
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 骨リモデリング / カップリング / 骨モデリング / イメージング / in vitro / 骨芽細胞 / 破骨細胞 / 骨細胞 |
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| Outline of Final Research Achievements |
To elucidate the interaction of cells which controls bone homeostasis, the environment in living body is reconstructed outside of the body, and a novel system was established to analyze it using 2-photon microscopy.
By culturing osteoblasts which form bones for long-term, and by observing same regions temporary, phenomena occurring during bone modeling such as increase of matrix, appearance of osteocytes which control bone metabolism, and changes in morphologies of osteoblasts on the matrix were reproduced and proved quantitatively.
In addition, in co-culture of long-term-cultured osteoblasts and osteoclasts which resorb bones, phenomena in bone remodeling such as matrix resorption by osteoclasts, appearance of mature osteoblasts in the resorption pits and replenishment of resorption pits could be captured at cellular level and quantified.
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| Free Research Field |
骨代謝、再生医療
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