Pathophysiology of life-related diseases with ectopic calcification: approaches for diagnostics and therapy

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670702
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: Kumon Hiromi 岡山大学, 医歯(薬)学総合研究科, 特命教授 (30144760)
• Co-Investigator(Kenkyū-buntansha): MATSUURA Eiji 岡山大学, 大学院医歯薬学総合研究科, 教授 (20181688) ; ARAKI Motoo 岡山大学, 病院, 講師 (90467746)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 石灰化小球 / 酸化脂質 / 質量分析 / イメージング質量分析 / 異所性石灰化

Outline of Final Research Achievements

Our study has revealed the components, especially, oxidized lipids derived from γ-irradiated FBS can act as a scaffold to generate so-called “Nanobacteria-like particles (NLP)”. Lipids were extracted from three different experimental groups and were subjected to be separated on TLC plate. Individual spot was collected and further analyzed with MALDI-TOF-MS, while the oxidized lipids were characterized with MS/MS. As a result, lyso-PC was detected in NLP grown in γ-irradiated FBS. Nevertheless, 7-ketocholesterol and oxLig-1 (7-ketocholesteryl 9-carboxynonanoate) were found abundantly in both γ-irradiated FBS itself and NLP grown with non-γ-irradiated FBS in the presence of oxLig-1-liposomes. Subsequent characterization of those NLP specimens by scanning electron microscopy revealed that NLPs grown in γ-irradiated FBS demonstrated rounded shapes with larger dimensions than those in non-γ-irradiated FBS.

Free Research Field

泌尿器科学、ナノバイオテクノロジー、遺伝子治療学