Genetic and epigenetic evaluation method for the sperm

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670715
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KOHDA Takashi 東京医科歯科大学, 難治疾患研究所, 准教授 (60211893)
• Co-Investigator(Kenkyū-buntansha): KUBOTA Toshiro 東京医科歯科大学, 医歯学総合研究科, 教授 (50126223) ; HARDA Tatsuya 東京医科歯科大学, 医歯学総合研究科, 講師 (80376748)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: sperm / hydroxymethylcytosine / ART

Outline of Final Research Achievements

In the assisted reproductive technology (ART), the identification method for “good” sperm has been needed. The morphological standard is not necessarily sufficient to identify the genetic and epigenetic integrity of the sperm genome. Here we apply the newly developed method that identify the methylcytosine, hydroxymethylcytosine and non-modified cytosine simultaneously to the single sperm. After the requirement study, we have successfully apply this method for the L1 repetitive sequence analysis for cytosine modification.

Free Research Field

分子生物学