Unravelling the mechanism of ovarian carcinogenesis caused by BRCA1 depletion and estrogen action

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670730
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: OHTA Tomohiko 聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (60233136)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: BRCA1 / エストロゲン / 卵管癌 / DNA損傷 / ヘテロクロマチン

Outline of Final Research Achievements

The mechanism of tissue specificity; how BRCA1 mutation specifically triggers only breast and ovarian cancer, has been unrevealed. In this study we established doxycyclin-inducible BRCA1 deficient and estrogen receptor α (ERα) positive diploid human breast cell lines and investigated the effect of estrogen receptor expression on genomic and epigenomic stability in BRCA1-defective cellular background. BRCA1-depleted/ERα-positive cells exhibited chromatin de-compaction, increase of G2/M phase population and apoptosis, accompanied by significant cell number reduction, in response to doxycyclin treatment when compared to control BRCA1-depleted or ERα-positive cells.

Free Research Field

産婦人科学