2016 Fiscal Year Final Research Report
Neuroprotective gene therapy for glaucoma model animals
| Project/Area Number |
26670756
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 緑内障 / 遺伝子治療 / 神経保護 |
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| Outline of Final Research Achievements |
In the present study, we used DBA/2J mice as a glaucoma model. First, we measured intraocular pressure (IOP) in DBA/2J mice at several time points and we checked IOP was high in almost all DBA/2J. Moreover, we performed histopathological analysis using retinal sections and flat-mount retinal specimens and functional gene expression analysis in retina, at several time points (two, 4, 6, 8, and 10 months after birth).
We also performed gene therapy study using 2-month-old DBA/2J mice. Now, we assess the therapeutic efficacy via retrograde labeling analysis of retinal ganglion cell.
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| Free Research Field |
眼科学
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