Analysis of inhibition of MRSA infection by quorum sensing molecules from pseudomonas aeruginosa

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670768
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Tohoku University
• Principal Investigator: Tachi Masahiro 東北大学, 医学(系)研究科(研究院), 教授 (50312004)
• Co-Investigator(Kenkyū-buntansha): KANNO Emi 東北大学, 医学系研究科, 講師 (10431595) ; TAKEDA Atsushi 東北大学, 医学系研究科, 助教 (30333800)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 緑膿菌 / クオラムセンシング分子 / MRSA / 感染

Outline of Final Research Achievements

We addressed the possible involvement of quorum sensing (QS) molecules, 3-oxo-C12-HSL and C4-HSL, from Pseudomonas aeruginosa to inhibition of methicillin-resistant Staphylococcus aureus (MRSA). We used Vancomycin (VCM) as a positive control. More than 10% Dimethyl sulfoxide (DMSO) had the effect of inhibition of MRSA, but on the other hand QS molecules had not that effect. In addition, the MRSA growth did not affect by the stimulation of 3-oxo-C12-HSL and
C4-HSL at the same time. Minimum inhibitory concentration of VCM showed 1 μg/ml. These results suggest that QS molecules, 3-oxo-C12-HSL and C4-HSL, have not effect to inhibition of MRSA.

Free Research Field

形成外科学、創傷治癒学