2015 Fiscal Year Final Research Report
Contribution of stiffness of extracellular matrix to pathogenesis of keloid
| Project/Area Number |
26670777
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | メカノトランスダクション |
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| Outline of Final Research Achievements |
① We assayed subcellular localization of YAP, which was recently identified as a sensor and a mediator of mechanical signals, in human dermal fibroblasts grown on collagen-type 1 coated-hydrogels on varying stiffness, and confirmed YAP was nuclear on hard substrates but became predominantly cytoplasmic on soft substrates.
② We identified nuclear translocation of YAP in human keloids but not normal white scars and nomad skins with immunohistochemical staining.
③ Gene expressions of extracellular matrix on hard substrates were higher in keloid-derived fibroblasts than white scar-derived fibroblasts and normal skin-derived fibroblasts.
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| Free Research Field |
形成外科学
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