Mechanisms of influenza virus mRNA nuclear export

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670781
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Akita University
• Principal Investigator: Imai Yumiko 秋田大学, 医学(系)研究科(研究院), 教授 (50231163)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: インフルエンザウイルス / RNA核外輸送

Outline of Final Research Achievements

NXF1 is previously known as a transporter of host mRNA. Using n vitro RNA gel-shift assay, we found that influenza virus mRNA directly binds to NXF1. In addition, we found that influenza virus mRNA can bind to MC domain of NXF1, although host mRNA binds to RBD, suggesting that binding patterns of influenza virus RNA and host RNA to NXF1 are different. Also, we developed a experimental system that can screen the compounds that can inhibit the interaction of viral mRNA and NXF1 MC domain. This may lead to develop the drugs that can inhibit influenza virus mRNA specific nuclear export.

Free Research Field

ウイルス学