Development of a novel mouse model that recapitulates bone metastasis seen in human.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670806
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Osaka University
• Principal Investigator: Yoneda Toshiyuki 大阪大学, 歯学研究科(研究院), 招へい教員 (80142313)
• Co-Investigator(Renkei-kenkyūsha): HATA Kenji 大阪大学, 大学院歯学研究科, 准教授 (80444496)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 骨転移 / 動物モデル

Outline of Final Research Achievements

Many types of cancers preferentially spread to bone, developing bone metastases. To develop novel therapeutic interventions for bone metastasis and to test their effectiveness, establishment of pre-clinical animal models that resemble bone metastasis occurring in cancer patients has been awaited. Here, we attempted to develop a novel mouse model that recapitulates the entire steps of bone metastasis seen in human. The MMTV-Cdh11-2A-ffLuc transgenic (Tg) vectors were designed and constructed. The ffLuc is a novel chemiluminescent/fluorescent protein that enables the dynamic imaging of bone metastasis with high resolution. The linealized Tg vectors were then injected into fertilized eggs of FVB mice. Our next plan will be to generate double Tg mice by crossing MMTV-neu mice and MMTV-Cdh11-2A-ffLuc mice and monitor bone metastases spontaneously developed in these mice by in vivo imaging. This mouse model will be a useful tool to understand the mechanism underlying cancer bone metastasis.

Free Research Field

生化学