How is the formation site of osteoclasts determined?

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670814
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: Takahashi Naoyuki 松本歯科大学, 総合歯科医学研究所, 教授 (90119222)
• Co-Investigator(Kenkyū-buntansha): Nakamichi Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829) ; Mizoguchi Toshihide 松本歯科大学, 総合歯科医学研究所, 講師 (90329475) ; Hosoya Akihiro 松本歯科大学, 歯学部, 講師 (70350824) ; Kobayashi Yasuhiro 松本歯科大学, 総合歯科医学研究所, 教授 (20264252)
• Co-Investigator(Renkei-kenkyūsha): Kawahara Ichiro 松本歯科大学, 歯学部, 教授 (20319114) ; Udagawa Nobuyuki 松本歯科大学, 歯学部, 教授 (70245801)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 破骨細胞 / 骨芽細胞 / 破骨細胞前駆細胞 / RANKL / M-CSF

Outline of Final Research Achievements

Why do osteoclasts formed only in the bone? It has been considered “ because osteoblasts express osteoclast differentiation factor RANKL and M-CSF." However, when RANKL and M-CSF are administered intraperitoneally to RANKL-deficient mice and M-CSF-deficient op/op mice, respectively, osteoclasts appear only in bone tissue. This indicates that expression sites of RANKL and M-CSF do not determine the formation site of osteoclasts. In the present study, we showed that osteoclast precursors (OP) which highly express RANK (RANK-abundant OP, rOP) are detected only in the bone tissue. Localization of rOP is shown to determine the formation site of osteoclasts.

Free Research Field

生化学