2015 Fiscal Year Final Research Report
Development of an in vitro model of oral lichen planus by utilizing a characteristic of immune-privilege
Project/Area Number |
26670817
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Pathobiological dentistry/Dental radiology
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Research Institution | Niigata University |
Principal Investigator |
IZUMI KENJI 新潟大学, 医歯学系, 教授 (80242436)
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Co-Investigator(Kenkyū-buntansha) |
ITO AKIKO 新潟大学, 医歯学総合病院, 講師 (80361898)
AKIBA YOSUKE 新潟大学, 医歯学総合病院, 講師 (70547512)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Keywords | 口腔扁平苔癬 / 口腔粘膜上皮細胞 / CD8陽性T細胞 / インビトロモデル / 共培養 / 免疫特権 |
Outline of Final Research Achievements |
We detected oral mucosa keratinocytes cultured in a tissue culture dish do not express FasL by immunocytochemistry, FACS and RT-PCR analyses. The pharmacological induction of FasL expression was not successful, either. This resulted in the difficulty in developing an in vitro model of oral lichen planus by utilizing a characteristic of immune-privilege. However, we found that approximately 10% of human T cells expressed FasL. This suggestied it may be possible to analyze the T cell-oral keratinocyte pathophysiological interation in vitro.
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Free Research Field |
再生歯学
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