2016 Fiscal Year Final Research Report
Construction of mesenchymal stem cells-calcium phosphate hybrid spheroids using an oxygen-permeable cell culture device
| Project/Area Number |
26670846
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 生体材料学 / 細胞培養デバイス |
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| Outline of Final Research Achievements |
Three-dimensional (3D) cellular constructs have been paid attention in the field of tissue engineering. Increase in size of cellular aggregates causes a depletion of oxygen in the core of aggregates. We have developed an oxygen-permeable cell culture device to prevent hypoxia in the core of the cellular aggregates. The aim of this study was construction of 3D cellular structure consisted of mesenchymal stem cells (MSCs) and calcium phosphate materials based on the oxygen-permeable device we developed. We found that the device was able to improve osteoblastic differentiation of MSCs and generate hybrid spheroids consisted of cells and calcium phosphates. These results suggest that the device is useful for engineering 3D cellular constructs for tissue engineering.
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| Free Research Field |
再生医工学
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