2016 Fiscal Year Final Research Report
Establishment of novel diagnostic method in oral candidiasis using LH-PCR and next-generation sequencing
| Project/Area Number |
26670868
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 口腔カンジダ症 / 次世代シーケンサー / LH-PCR法 / 診断方法 / 加齢 |
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| Outline of Final Research Achievements |
The widespread use of empiric and prophylactic antifungal drugs has caused a shift in fungal biodiversity towards other Candida. We thus used NGS to examine the oral fungal biodiversity of 27 patients with pseudomembranous oral candidiasis (POC) and 66 healthy controls. The total number of fungal species in patients with POC and healthy controls was 67 and 86, respectively. The copy number of total PCR products and the proportion of non-C. albicans, especially C. dubliniensis, in patients with POC, were higher than those in healthy controls. The detection patterns in patients with POC were similar to those in controls after antifungal treatment. Interestingly, the number of fungal species and the copy number of total PCR products in healthy controls increased with aging. These results suggest that high fungal biodiversity and aging might be involved in the pathogenesis of oral candidiasis. We therefore conclude that NGS is a useful technique for investigating oral candida infections.
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| Free Research Field |
口腔外科学
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