Functional miRNA promotes the bone regeneration with rhBMP2 and ADCs

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670871
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University
• Principal Investigator: TAJIMA Nobutaka 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (00447492)
• Co-Investigator(Kenkyū-buntansha): ASAHINA Izumi 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039) ; SUMITA Yoshinori 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 骨再生 / 脂肪由来細胞 / microRNA

Outline of Final Research Achievements

Direct implantation of freshly isolated adipose-derived cells (ADC) with minimal amount of rhBMP2 to the mouse cranium were performed to assess the effectiveness of administration of functional miRNA in vivo. On the other hand, mouse adipose mesenchymal stem cells (AD-MSC) were exposed to rhBMP2 for osteoblastic induction in culture to search the candidates of miRNAs. As a result, when a certain miRNA, which were chosen in vitro assay, were transplanted with ADC and minimal amount of BMP2, we found this miRNA can accelerate the new bone formation. At present, we are doing further experiments to investigate the function of this miRNA in detail.

Free Research Field

口腔外科学・再生医学