2016 Fiscal Year Final Research Report
Biological study on ameloblastin based on evolutionary medicine
| Project/Area Number |
26670890
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | アメロブラスチン / AMBN / 進化医学 |
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| Outline of Final Research Achievements |
Ameloblastin (AMBN) is an enamel specific protein that plays critical roles in enamel formation. Expression of Xenopus laevis (X. laevis) AMBN mRNA was observed only in ameloblasts. An immunohistochemical study showed the distribution of the N-terminal peptides of X. laevis AMBN was different from that of the C-terminal peptides during enamel formation. The results indicated that X. laevis AMBN was an ameloblast-specific matrix protein that may initially be cleaved into two groups, N- and C-terminal peptides, as shown in amniotes. Consequently, it is suggested that AMBN have kept its original cleavage mechanism and N- and C-terminal peptides have served the respective different functions during the evolutionary transition from amphibian to amniote. It might have been conserved in both lineages during 350 million years of evolution.
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| Free Research Field |
小児歯科学
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