2016 Fiscal Year Final Research Report
Development of regeneration therapy by mesenchymal stem cell from gnathic bone marrow using novel nonwoven fabric filter
| Project/Area Number |
26670894
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Meikai University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 間葉系細胞 / 顎骨 / 不織布フィルター / 再生療法 |
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| Outline of Final Research Achievements |
The purpose of this study was to evaluate the usefulness of a device with a nonwoven fabric filter, which selectively traps MSC based on affinity, for isolation of MSC from gnathic bone marrow. A total of 44 bone marrow aspirates were obtained from 35 patients during surgery for dental implant placement. The isolated cells were then seeded on tissue culture dishes. As a control, without using the device, the cells harvested by centrifugation were directly seeded on the dishes. As a result, colony forming cells by using the device were detected in 10 of 30 samples (33.3%). The colony forming cells without using the device were obtained in 3 of 14 samples (21.4%). It was thus suggested that the use of the device with a nonwoven fabric filter may allow efficient isolation of MSC from gnathic bone marrow in a closed system.
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| Free Research Field |
歯周病学
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