2015 Fiscal Year Final Research Report
Comprehensive analysis of protein in gingival crevicular fluids from deciduous and permanent teeth using LC-ESI-MS/MS quantitative proteomics
| Project/Area Number |
26670896
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Periodontology
|
|---|
| Research Institution | Showa University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
INOUE Mitsuko 昭和大学, 歯学部, 客員教授 (20112724)
ITABE Hiroyuki 昭和大学, 薬学部, 教授 (30203079)
KOIDE Yoko 昭和大学, 歯学部, 助教 (40407466)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 歯肉溝滲出液 / 乳歯 / 永久歯 / 好中球 / 質量分析 |
|---|
| Outline of Final Research Achievements |
To identify the biochemical characteristics of gingival tissues for distinguishing between permanent and deciduous teeth, the protein profiles of gingival crevicular fluids (GCF) were analyzed using quantitative proteomics. Forty children with mixed dentition (Hellman’s dental age IIIA) were selected and their GCF samples were collected from both deciduous cuspids and central incisors in the maxilla. Among them, neutrophil-delived proteins, myeloperoxidase and lactoferrin, were upregulated in deciduous GCF. Protein levels of myeloperoxidase and lactoferrin in individual GCF samples, again determined by ELISA, were significantly higher in deciduous GCF than in permanent GCF (P < 0.01), confirming the predominance of neutrophil-derived proteins in deciduous GCF. These findings suggest that neutrophil accumulation is beneficial for gingival tissue of deciduous teeth and shows difference of protective mechanism against bacterial infection between deciduous and permanent teeth.
|
| Free Research Field |
歯周病態・診断学
|
