Elucidation of the oral biofilm prevention mechanism by photocatalytic devices and their practical application

2016 Fiscal Year Annual Research Report

• Project/Area Number: 26670901
• Research Institution: University of Tsukuba
• Principal Investigator: 楊 英男 筑波大学, 生命環境系, 教授 (50561007)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 光触媒デバイス / バイオフィルム / 口腔衛生 / 反応機構

Outline of Annual Research Achievements

光により励起される光触媒の酸化還元力や超親水性の発現などを医学や医療に関連する周辺領域への応用の可能性を探り、実用化を検討することは光機能材料の持つ能力を有効に引出し、安全で豊かな環境の創造に役立つ重要な研究と考えられている。そこで、本研究では安全で耐久性に優れた光触媒膜の製作、またそれを用いて口腔内バイオフィルムと光触媒の相互作用によるバイオフィルム生成の抑制効果の検討及び活性種の生成とその同定を行うことによって、光触媒を用いたバイオフィルム処理のメカニズムを明らかにする。さらに、本材料を利用して、口腔内バイオフィルム抑制デバイスを作製し、実用化に向けての実証を目的とする。
本研究室が開発したP/Ag2O/Ag/Ag3PO4/TiO2新規光触媒材料をもとに、耐久性が優れているPEG修飾したP/Ag2O/Ag/Ag3PO4-TiO2新規光触媒材料を開発した。その新規光触媒材料は、最適割合条件で従来の材料に比べ高い光触媒活性と耐久性を持つことが確認できた。H28年度は、前年度開発したP/Ag2O/Ag/Ag3PO4/TiO2光触媒材料をもとに、さらに光活性の高いAg/Ag2O/BiPO4/Bi2WO6新規光触媒材料を開発した。本材料を用いて殺菌効果を検討し、108CFU/mlの大腸菌が30分以内で完全死滅したとの大変優れた結果が得られた。
さらに、材料への最適コーティング条件と反応機構を解明したことによって、デバイスの作成試みた。口腔内から採集したバイオフィルムの培養を行い、人工歯型を用いて、開発した材料によるバイオフィルム抑制効果を検討した結果、十分な殺菌効果があることが明らかになった。今後の実用化に向け貴重な情報を得られた。
この一年間の研究成果は国際誌4本登載、特許１本、国際学会9件、国内学会9件の実績が得られた。

Research Products

• [Journal Article] Influence of cell size on cellular uptake of gold nanoparticles (2016)
  • Author(s): Wang, X., Hu, X., Li, J., Adriana C. Mulero Russe, Kawazoe, N., Yang, Y. N. Chen, G
  • Journal Title: Biomaterial Science Volume: 4 Pages: 970-978
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Manipulating cell nanomechanics using micropatterned surfaces (2016)
  • Author(s): Wang, X., Hu, X., Kawazoe, N., Yang, Y. N. Chen, G.
  • Journal Title: Advanced Functional Materials Volume: 26 Pages: 7634-7643
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Morphological and mechanical properties of osteosarcoma microenvironment cells explored by atomic force microscopy (2016)
  • Author(s): 8.Wang, X., Yang, Y. J., Hu, X., Kawazoe, N., Yang, Y. N. Chen, G.
  • Journal Title: Analytical Sciences Volume: 32 Pages: 1177-1182
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] An alternative anaerobic treatment process for treatment of heavy oil refinery wastewater containing polar organics. (2016)
  • Author(s): Wang, Y., Wang, Q., Li, M., Yang, Y. N., He, W., Yan, G., Guo, S.
  • Journal Title: Biochemical Engineering Journal Volume: 105 Pages: 44-51
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Development of Highly Efficient Visibal-light-driven P/Ag/Ag2O/Ag3PO4/TiO2 Photocatalyst (2017)
  • Author(s): Yang, Y. N.
  • Organizer: Shanghai University International Cooperation Symposium
  • Place of Presentation: Shanghai University, Shanghai, China
  • Year and Date: 2017-03-16
  • Int'l Joint Research / Invited
• [Presentation] Enhanced bactericidal activity of Escherichia coli with a novel TiO2 based composite synthesized by a hydrothermal method (2017)
  • Author(s): Liu, N., Zhu, Q., Negishi, N., Yang, Y. N.
  • Organizer: つくば医工連携フォーラム2017
  • Place of Presentation: 国立法人物質材料研究機構千現地区、茨城県つくば
  • Year and Date: 2017-01-20
• [Presentation] Preparation of Ag-Based Bi2WO6 Thin Film with Enhanced Photocatalytic Activity for Degradation of Wastewater (2017)
  • Author(s): Ma, Q. S., Hu, X. H., Yang, Y. N.
  • Organizer: つくば医工連携フォーラム2017
  • Place of Presentation: 国立法人物質材料研究機構千現地区、茨城県つくば
  • Year and Date: 2017-01-20
• [Presentation] Comparing the Water Splitting Efficiency of Photocatalytic Composite Prepared Under Different Calcination Temperature by Hydrothermal Method (2017)
  • Author(s): Nagai, D., Zhu, Q., Yang, Y. N.
  • Organizer: つくば医工連携フォーラム2017
  • Place of Presentation: 国立法人物質材料研究機構千現地区、茨城県つくば
  • Year and Date: 2017-01-20
• [Presentation] Physico-chemical Properties of Visible Light-responsive TiO2-based Nanophotocatalyst with Silver Dopant and Improved Photocatalytic Ability by Hydrothermal Method. (2017)
  • Author(s): Zhu, Q., Hu, X. H., Stanislaus, M.S., Yang, Y. N
  • Organizer: つくば医工連携フォーラム2017
  • Place of Presentation: 国立法人物質材料研究機構千現地区、茨城県つくば
  • Year and Date: 2017-01-20
• [Presentation] Photodynamic therapy for cancer cells by TiO2 based nano photosensertizer (2017)
  • Author(s): Hu, X. H., Wang X. L., Zhu, Q., Zhang, N., Kawazoe N., Yang, Y. N.
  • Organizer: つくば医工連携フォーラム2017
  • Place of Presentation: 国立法人物質材料研究機構千現地区、茨城県つくば
  • Year and Date: 2017-01-20
• [Presentation] 可視光利用型新規光触媒材料の研究開発 (2016)
  • Author(s): 楊 英男
  • Organizer: 第14回環境研究シンポジウム
  • Place of Presentation: 一橋大学、東京都、千代田区
  • Year and Date: 2016-11-22
• [Presentation] Development of Highly Efficient Visibal-light-driven TiO2 Photocatalyst (2016)
  • Author(s): Yang, Y. N., Hu, X. H., Zhang, N., Stanislaus, M.S.
  • Organizer: The 12th IUPAC International Conference on Novel Materials and their Synthesis (NMS-XII)
  • Place of Presentation: Changsha, China
  • Year and Date: 2016-10-14 – 2016-10-16
  • Int'l Joint Research / Invited
• [Presentation] Improvement of silver modified TiO2 solar-light-driven photocatalyst by hydrothermal method (2016)
  • Author(s): Zhu, Q., Hu, X. H., Mishma, S. S., Yang, Y. N.
  • Organizer: 68th SBJ Annual Meeting
  • Place of Presentation: Toyama International Conference Center,ANA Crowne Plaza Toyama, Toyama, Toyama-city
  • Year and Date: 2016-09-28 – 2016-09-30
• [Presentation] Inactivation of Escherichia coli using P/Ag/Ag2O/Ag3PO4/TiO2 photocatalyst under LED irradiation (2016)
  • Author(s): Liu, N., Zhu, Q., Xiao, R. D., Yang, Y. N.
  • Organizer: 68th SBJ Annual Meeting
  • Place of Presentation: Toyama International Conference Center,ANA Crowne Plaza Toyama, Toyama, Toyama-city
  • Year and Date: 2016-09-28 – 2016-09-30
• [Presentation] Development of P/Ag/Ag2O/Ag3PO4/TiO2 thin film modified by polyethylene glycol for water treatment (2016)
  • Author(s): Ma, Q. S., Dong, Z. T., Zhu, Q., Hu, X. H., Yang, Y. N.
  • Organizer: 68th SBJ Annual Meeting
  • Place of Presentation: Toyama International Conference Center,ANA Crowne Plaza Toyama, Toyama, Toyama-city
  • Year and Date: 2016-09-28 – 2016-09-30
• [Presentation] Effect of Hydrothermal Treatment on Physico-chemical Properties of Visible Light-responsive P/Ag/Ag2O/Ag3PO4/TiO2 Photocatalyst (2016)
  • Author(s): Zhu, Q., Hu, X. H., Stanislaus, M.S., Yang, Y. N.
  • Organizer: The 9th Korea-China-Japan Graduate Student Forum
  • Place of Presentation: Chungnam National University,Daejeon, Korea
  • Year and Date: 2016-09-20 – 2016-09-23
  • Int'l Joint Research
• [Presentation] Effects of experiment conditions on the inactivation of Escherichia coli using P/Ag/Ag2O/Ag3PO4/TiO2 photocatalyst under LED irradiation. (2016)
  • Author(s): Liu, N., Zhu, Q., Negishi, N., Yang, Y. N.
  • Organizer: Ag-ESD Symposium 2016
  • Place of Presentation: University of Tsukuba, Tsukuba, Ibaraki, Japan
  • Year and Date: 2016-09-18 – 2016-09-19
  • Int'l Joint Research
• [Presentation] Enhancement of P/Ag/Ag2O/Ag3PO4/TiO2 Solar-light-driven Photocatalyst by Hydrothermal Method (2016)
  • Author(s): Zhu, Q., Hu, X. H., Stanislaus, M.S., Yang, Y. N.
  • Organizer: Ag-ESD Symposium 2016
  • Place of Presentation: University of Tsukuba,Tsukuba, Ibaraki, Japan
  • Year and Date: 2016-09-18 – 2016-09-19
  • Int'l Joint Research
• [Presentation] Synthesization of PEG Modified P/Ag/Ag2O/Ag3PO4/TiO2 Photocatalyst (2016)
  • Author(s): Dong, Z. T., Zhu, Q., Hu, X. H., Yang, Y. N.
  • Organizer: Ag-ESD Symposium 2016
  • Place of Presentation: University of Tsukuba,Tsukuba, Ibaraki, Japan
  • Year and Date: 2016-09-18 – 2016-09-19
  • Int'l Joint Research
• [Presentation] Optimization of H2 and O2 Generation in Water-splitting Process by P/Ag/Ag2O/Ag3PO4/TiO2 photocatalyst under Solar Light (2016)
  • Author(s): Hu, X. H., Shen, H. X., Zhu, Q., Yang, Y. N.
  • Organizer: Ag-ESD Symposium 2016
  • Place of Presentation: University of Tsukuba,Tsukuba, Ibaraki, Japan
  • Year and Date: 2016-09-18 – 2016-09-19
  • Int'l Joint Research
• [Presentation] Preparation of Ag-based Bi2WO6 Thin Film on Glass Substrate with Enhanced Photocatalytic Activity for Decomposition of Organic Dye (2016)
  • Author(s): Ma, Q. S., Hu, X. H., Zhu, Q., Yang, Y. N.
  • Organizer: Ag-ESD Symposium 2016
  • Place of Presentation: University of Tsukuba,Tsukuba, Ibaraki, Japan
  • Year and Date: 2016-09-18 – 2016-09-19
  • Int'l Joint Research
• [Presentation] Comparing the Water Splitting Efficiency by Using Different Temperature of Hydrothermal Synthesis of Photocatalytic Composite Under Solar Light (2016)
  • Author(s): Nagai, D., Zhu, Q., Shen, H. X., Yang, Y. N.
  • Organizer: Ag-ESD Symposium 2016
  • Place of Presentation: University of Tsukuba, Tsukuba, Ibaraki, Japan
  • Year and Date: 2016-09-18 – 2016-09-19
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] 可視光で利用できる光触媒 (2016)
  • Inventor(s): 楊英男、呼小紅
  • Industrial Property Rights Holder: 楊英男、呼小紅
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2016-218918
  • Filing Date: 2016-11-09