Development of fast detection system for oral microorganism which aims clinical application in infectious oral deiseases

2016 Fiscal Year Final Research Report

• Project/Area Number: 26670903
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: Nagata Hideki 大阪大学, 歯学研究科, 招へい教員 (50260641)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 予防歯科学 / 口腔微生物 / 検出システム / 歯周病細菌 / PCR

Outline of Final Research Achievements

It is important to examine kinds and quantity of microorganism associated with oral infectious diseases for diagnosis, effeicacy of treatment, and prognosis. In this study, we developed the detection system with capillary polymer electrophoresis, and its sensitivity and quantity to detect oral microorganism were analyzed. Sensitivity and quantity of the detection system was equal to those of real-time PCR. The ease and the high sensitivity of the system to detetect periodontopathic bacteria were illustrated by analyzing clinical saliva and plaque samples. Moreover, the syetem discriminated the type of Porphyromonas gingivalis fimbriae.

Free Research Field

予防歯科学