Analysis of pseudo transcription formation following transcription remodeling leading to homologous recombination repair

2017 Fiscal Year Final Research Report

• Project/Area Number: 26701005
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Gunma University
• Principal Investigator: Shibata Atsushi 群馬大学, 大学院医学系研究科, 助教 (30707633)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: DNA修復 / Homologous recombination / NHEJ / クロマチン / 転写

Outline of Final Research Achievements

Ionizing irradiation causes DNA double strand breaks, which can lead to cell death if unrepaired or misrepaired. In the present study, to identify a novel factor promoting HR at the step of DSB end resection, we carried out a screening experiment. Importantly, our analysis demonstrated that novel HR factors, MEC1 and REC1, which are originally involved in transcription, promote the initiation of HR by activating the step of DSB end resection. This finding provides us important information regarding the maintenance of genome stability after ionizing irradiation.

Free Research Field

分子生物学